G4

product
  • ER
  • AR
  • PI3K
  • MAPK

*OncoSignal is available for Research Use Only – not for use in diagnostic procedures.

Developed under ISO13485.

The OncoSIGNal G4 for measuring ER, AR, PI3K and MAPK pathway activity is available for research use in your molecular biology laboratory. The test is performed using an RT-qPCR testing plate to measure mRNA levels of selected pathway target genes and includes access to a secure cloud-based environment for calculation and reporting of pathway activity scores.

OncoSignal is based on a unique knowledge-based approach and helps users gain insight into underlying tumor driving cell signaling pathways. The test quantitatively measures the activity of the hormonal estrogen receptor (ER) and androgen receptor (AR) pathways, as well as the activity of PI3K and MAPK growth factor signal transduction pathways simultaneously in a single sample, using RNA from FFPE tissue of human origin.

 

Features
Allows for in-house testing using standard laboratory equipment

  • AR, ER, PI3K, MAPK pathway activity
  • Highly reproducible quantitative pathway activity scores on a scale of 0-100 with confidence intervals
  • Translation of RT-qPCR data into pathway activity scores in a secure cloud-based environment
  • Report providing pathway activity profile per sample
  • Easy to implement in molecular biology laboratory
  • Requires only 0.25 mm3 FFPE tissue per sample
  • Fast turn-around-time (105 minutes)

Workflow
Easy to integrate into laboratory workflow

01

Select FFPE tumor tissue block

02

Identify tumor area

03

Macro dissect tumor area from unstained slide(s)

04

Transfer tissue to RNase-free tube

05

Add PCR mix with extracted RNA to testing plate

06

Run the test on a thermocycler

07

Process PCR data in cloud-based environment

08

Generate report with pathway activity scores

Box contains: • Six testing plates (96-wells plates with pre-designed and spotted qPCR assays)
• Six (+one spare) clear, adhesive PCR plate foils
• Quick Reference Guide
Additional reagents and equipment needed: • RNeasy FFPE Kit (Qiagen, cat. no. 73504)
• SuperScript III Platinum One-Step qRT-PCR Kit (Thermo Fisher Scientific, cat.no. 11732088)
• Bio-Rad CFX96 (Touch) Real-Time PCR Detection System
• Plate centrifuge

For information about example experimental designs, and interpretation of the OncoSIGNal Report, please request the application note.

Contact us

E-Mail us oncosignal@innosignbio.com

 

A number of cell signal transduction pathways are known to play a role in the development of cancer. OncoSIGNal helps to gain insight into the functional activity of these underlying tumor driving signaling pathways. mRNA levels transcribed from direct target genes regulated by the pathway transcription factors are measured and translated into a quantitative pathway activity scores. The activity score of each pathway is reported on a scale from 0 to 100, resulting in a quantitative characterization of the cell molecular phenotype.

How can OncoSIGNal benefit you?

  • Unique insights

    Profiling of pathway activity by OncoSIGNal provides new insights into the molecular mechanisms of cancer. For each cell and tissue sample the activity scores of the signaling pathways are reported.

     

  • Tailored to your needs

    OncoSIGNal is available as PCR test for decentralized use, RNA-seq data analysis service, and service testing in our EU lab.

  • All tumor types

    OncoSIGNal products are developed based on deep insights in cell-biology using a knowledge-based approach. This enables application of the tests for a wide variety of tumor tissue types.

Products & Services

The test is performed using an RT-qPCR testing plate to measure mRNA levels of selected pathway target genes and includes access to a secure cloud-based environment for calculation and reporting of pathway activity scores. The OncoSignal Test has been developed under ISO13485.

 

*OncoSignal is available for Research Use Only – not for use in diagnostic procedures.

OncoSIGNal Report

The OncoSIGNal portal supports an export function of OncoSIGNal test results to Excel that contains the Pathway Activity Profiling OncoSIGNal scores of multiple samples. The report export shows all sample information, flags, test status and OncoSIGNal scores with corresponding upper and lower confidence intervals. This report export will enable further data analysis or data visualization or the report can be adjusted and sorted to the user’s preference.

This example of the OncoSIGNal Report (generated by using the batch export option) shows how the pathway activity scores may be used in a retrospective study. The goal of the study was to investigate if OncoSIGNal can help to explain response to neo-adjuvant hormonal therapy; the retrospective study included patient samples of responders and non-responders to aromatase inhibitor (AI) treatment. Biopsy tissue from estrogen receptor (ER) IHC positive patients before start of neo-adjuvant and after neo-adjuvant AI treatment were measured. The largest differences in pathway activity can be observed for the ER pathway. In general, the relatively high ER pathway activity scores obtained from the pre-treatment samples decreased upon treatment, which might be an indication of successful AI treatment. Patient samples 102373 + 102374 however, show a relatively low pre-treatment ER activity score indicating a lower ER pathway activity before treatment, which may explain why this patient did not benefit from the AI treatment. Studying the pathway activity profile may provide further direction to identify relevant tumor driving pathways, such as the relatively high MAPK activity measured for patient samples 102373 + 102374 and relatively high PI3K activity for patient samples 102369 + 102370, respectively.