Service Testing

service

*OncoSIGNal is available for Research Use Only – not for use in diagnostic procedures.

ISO13485 certified.

OncoSIGNal Service Testing is offered via the InnoSIGN ISO13485 certified service lab at the High Tech Campus Eindhoven, the Netherlands.

Features

Compatible with FFPE tissue samples and RNA extracted from cells and tissue of human origin
– Testing of FFPE samples, providing Pathway Activity Scores for ER, AR, PI3K, MAPK, Hedgehog, Notch, TGFβ, Wnt, and JAK-STAT1/2,
pathways for each sample (includes tissue scraping from FFPE slides, RNA extraction, RT-qPCR for detection of all relevant target genes, pathway activity analysis and reporting).
– Testing of RNA samples, providing Pathway Activity Scores for the ER, AR, PI3K, MAPK, TGFβ, Hedgehog, Notch, TGFβ, Wnt and JAK-STAT1/2 pathways for each sample (includes RT-qPCR for detection of all relevant target genes, pathway activity analysis and reporting).
Extensive quality checks on samples, laboratory process and results
Report providing pathway activity profile per sample

Interested in Service Testing, please contact us.

More information

Email oncosignal@innosignbio.com

How can OncoSIGNal benefit you?

Unique insights

Profiling of pathway activity by OncoSIGNal provides new insights into the molecular mechanisms of cancer. For each cell and tissue sample the activity scores of the signaling pathways are reported.

Tailored to your needs

OncoSIGNal is available as PCR test for decentralized use, RNA-seq data analysis service, and service testing in our EU lab.

All tumor types

OncoSIGNal products are developed based on deep insights in cell-biology using a knowledge-based approach. This enables application of the tests for a wide variety of tumor tissue types.

Products & Services

The test is performed using an RT-qPCR testing plate to measure mRNA levels of selected pathway target genes and includes access to a secure cloud-based environment for calculation and reporting of pathway activity scores. The OncoSIGNal Test has been developed under ISO13485.

*OncoSIGNal is available for Research Use Only – not for use in diagnostic procedures.

OncoSIGNal Report

The OncoSIGNal portal supports an export function of OncoSIGNal test results to Excel that contains the Pathway Activity Profiling OncoSIGNal scores of multiple samples. The report export shows all sample information, flags, test status and OncoSIGNal scores with corresponding upper and lower confidence intervals. This report export will enable further data analysis or data visualization or the report can be adjusted and sorted to the user’s preference.

This example of the OncoSIGNal Report (generated by using the batch export option) shows how the pathway activity scores may be used in a retrospective study. The goal of the study was to investigate if OncoSIGNal can help to explain response to neo-adjuvant hormonal therapy; the retrospective study included patient samples of responders and non-responders to aromatase inhibitor (AI) treatment. Biopsy tissue from estrogen receptor (ER) IHC positive patients before start of neo-adjuvant and after neo-adjuvant AI treatment were measured. The largest differences in pathway activity can be observed for the ER pathway. In general, the relatively high ER pathway activity scores obtained from the pre-treatment samples decreased upon treatment, which might be an indication of successful AI treatment. Patient samples 102373 + 102374 however, show a relatively low pre-treatment ER activity score indicating a lower ER pathway activity before treatment, which may explain why this patient did not benefit from the AI treatment. Studying the pathway activity profile may provide further direction to identify relevant tumor driving pathways, such as the relatively high MAPK activity measured for patient samples 102373 + 102374 and relatively high PI3K activity for patient samples 102369 + 102370, respectively.

Service Testing